Epiderman growth factor (EGF), a protein of approximately 60kDa, contributes to a complex network of growth factors and receptors that together help to modulate cell growth, proliferation and differentiation. EGF is secreted by a variety of cells within the body. Following secretion, the protein influences other cells, or even the cell secreting it, by binding EGF receptors on the cell surface and in doing so activating tyrosine kinase signalling and transduction pathways within the cytoplasm. EGF is ideal for use in cell culture as it is mitogenic for a variety of epidermal and epithelial cells. These include fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells. The Effective Dose (ED50) of all SCIPAC EGF is determined by spiking EGF at varying concentrations into a cell proliferation assay using Blab/c 3T3 cells. This quality control procedure ensures EGF’s mitogenic activity is consistent from batch to batch.
| Abbreviation | EGF |
| Variant Name | Product Code | Purity | |
|---|---|---|---|
+ |
Epidermal Growth Factor, highly pure | T200-1 | Highly Pure |
